SmartNano

논문 초록 (Abstract)

We have developed a facile method for the poly(allylamine hydrochloride) (PAH)-assisted synthesis of mesoporous calcium silicate hydrates (PAH-CS) with a large specific surface area (BET = 348.4 m2 g−1) and pore volume (Vp = 1.42 cm3 g−1). Tetraethyl orthosilicate (TEOS) was employed as a silicon source, which was rapidly hydrolyzed and reacted with the amine groups of PAH to form spherical SiO2 nanoparticles (PAH-Si). Subsequently, Ca2+ ions reacted with the silicate anions produced during the dissolution of SiO2 in basic media, leading to the formation of the highly porous 3D networks of PAH-CS that were synthesized only under optimized reaction conditions. The PAH-CS containing an excess of Ca2+ and NH3+ enriched the surfaces with a very high cationic charge (ζ = +65.66 mV)and resulted in an extremely high loading capacity for anionic drugs and proteins. Ibuprofen (IBU) and FITC-labeled bovine albumin (FITC-Albumin) were chosen as a model drug and model protein, respectively, to test the loading and delivery efficiencies of the PAH-CS carriers. The ultrahigh drug loading capacities (DLC) and their release patterns were investigated under controlled pH conditions. Strikingly, the highest DLC reported to date (IBU or FITC-Albumin/carrier (3.35 g or 1 g g−1) was achieved in this work. The PAH-CS had no cytotoxic effect on osteoblast-like MC3T3-E1 cell lines evaluated by the LDH (Lactate dehydrogenase) assay in supernatant medium. Furthermore, the PAH-CS carriers could be entirely transformed to hydroxyapatite after releasing the drug in simulated body fluid (SBF), indicating good bioactivity and biodegradability of the PAH-CS carriers.