콘텐츠 본문
논문 해외 국제전문학술지(SCI급) Estimation of direct-contact fraction for phenanthrene in surfactant solutions by toxicity measurement
- 학술지 구분 국제전문학술지(SCI급)
- 게재년월 2007-09
- 저자명 우승한
- 학술지명 JOURNAL OF BIOTECHNOLOGY
- 발행처명 ELSEVIER SCIENCE BV
- 발행국가 해외
- 논문언어 외국어
- 전체저자수 5
논문 초록 (Abstract)
The toxicity of solutions containing nonionic surfactants Tween 80, Brij 35 and/or phenanthrene to Pseudomonas putida ATCC 17484 was investigate d. The fraction of direct contact between micellar-phase phenanthrene an d bacterial cell surface was estimated byusing the toxicity data and a mathematical model. The mathematical model was used to calculate phenan threne concentration in the micellar phase and aqueous pseudophase separ ately. The first-order death rate constant increased from 0.088 +/- 0.01 6 to 0.25 +/- 0.067 h(-1) when the phenanthrene concentration was increa sed from 0 to 5.17 x 10(-6) M (equals water solubility). The intrinsic t oxicity of surfactant was higher in Brij 35 than in Tween 80. When phena nthrene concentration was increased to 9.7 x 10(-5) M in surfactant solu tions, the death rate constant increased to 1.8 +/- 0.024 and 0.41 +/- 0 .088 h(-1) for 8.4 x 10(-4) M Brij 35 and 7.6 x 10(-4) M Tween 80. The d irect-contact fraction was 0.083 and 0.044 for Brij 35 and Tween 80, res pectively, under these conditions using exponential model. The toxicity increased with increasing phenanthrene concentration at a fixed surfact ant concentration. The toxicity decreased with increasing the surfactant concentration at a fixed phenanthrene concentration due to decreased co ntact of bacteria with phenanthrene present in the interior of surfactan t micelles. (c) 2007 Elsevier B.V All rights reserved.
