생물환경연구실

논문 초록 (Abstract)

Three different bacteria capable of degrading phenanthrene were isolated from sludge of a pulp wastewater treatment plantand identified as Acinetobacter baumannii, Klebsiella oxytoca, an d Stenotrophomonas maltophilia. Phenanthrene degradation effici encies by different combinations (consortia) of these bacteria were inv estigated and their population dynamics during phenanthrene degradation were monitored using capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP). When a single microorgan ism was used, phenanthrene degradation efficiency was very low (48.0, 11.0, and 9.0% for A baumannii, K. oxytoca, and S. maltophilia respecti vely, after 360h cultivation). All consortia that included S. malto philia degraded similar to 80.0% of phenanthrene and reduced lag tim e to 48 h compared to the 168 h of pure A. baumannii culture. CE-SSCP a nalysis showed that S. maltophilia was the predominant species during phenanthrene degradation in the mixed culture. The results in dicate that mixed cultures of microorganisms may effectively degrade target chemicals, even if the microorganisms show low degradation ac tivity in pure culture. (C) 2009 Elsevier B.V. All rights reserved.