콘텐츠 본문
논문 해외 국제전문학술지(SCI급) Cyclosporine A Reduces Dendritic Outgrowth of Neuroblasts in the Subgranular Zone of the Dentate Gyrus in C57BL/6 Mice
- 학술지 구분 국제전문학술지(SCI급)
- 게재년월 2010-03
- 저자명 박정호
- 학술지명 NEUROCHEMICAL RESEARCH
- 발행처명 SPRINGER/PLENUM PUBLISHERS
- 발행국가 해외
- 논문언어 외국어
- 전체저자수 10
논문 초록 (Abstract)
In the present study, we observed the effects of cyclosporine A (CsA), an efficient immunosuppressant, on cell proliferation andneuroblast differentiation in the subgranular zone of the dentate gyrus (SZDG) in normal C57BL/6 mice using Ki67 and doublecortin (DCX) immunohistochemical staining, respectively. At 8 week s of age, vehicle (physiological saline) orCsA was daily administered (40 mg/kg, i.p.) for 1 week. Animals were sacrificed at 2 weeks after last administration. CsA treatment did not show any influ ences in neurons, astrocytes and microglia based on immunohistochemist ry for its markers, respectively. However, in the CsA-treated group, Flu oro-Jade B, a marker for neurodegeneration, positive cells were fo und in the SZDG, not in the vehicle-treated group. In the vehicle-tre ated group, Ki67 immunoreactive ((+)) nuclei were clustered in the SZ DG, whereas in the CsA-treated group Ki67(+) nuclei were scattered in t he SZDG, showing no difference in cell numbers. Numbers of DCX+ neurobla sts with well-developed processes (tertiary dendrites) were m uch lower in the CsA-treated group than those in the vehicle-treated group; however, numbers of DCX+ neuroblasts with secondary dendrite s were similar in both the groups. These results suggest that CsA sig nificantly reduces dendritic outgrowth and complexity from neuroblasts in the SZDG without any affecting in neurons, astrocytes and microglia in normal mice.
